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以欧李“大磨盘”的茎尖为外植体,1/2MS和MS为基本培养基,研究了不同生长激素对启动培养的影响,不同培养基对丛生芽增殖的影响,初步建立了欧李“大磨盘”丛生芽的诱导及生根技术。适宜“大磨盘”的启动培养的生长素为IBA,其最佳丛生芽的培养基为MS+6-BA1.0mg/L+NAA0.01mg/L+IBA0.2mg/L,最佳分化培养基是MS+6-BA0.5mg/L+NAA0.01mg/L+IBA0.2mg/L,最佳生根培养基是1/2MS+IBA0.5mg/L+NAA0.1mg/L。
In order to study the effects of different growth hormones on starter culture and the effect of different media on the proliferation of cluster buds, the stem tips of OuLi “big disc” were used as explants, 1 / 2MS and MS as basic medium The European Lee “large disc ” clumping bud induction and rooting techniques. The optimal auxin for the “Macropod” culture was IBA. The optimal culture medium was MS + 6-BA 1.0 mg / L + NAA 0.01 mg / L + IBA 0.2 mg / L, and the best differentiation The medium was MS + 6-BA0.5mg / L + NAA0.01mg / L + IBA0.2mg / L, the best rooting medium was1 / 2MS + IBA0.5mg / L + NAA0.1mg / L.