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采用大鼠原代肝细胞ＵＤＳ试验研究了广西某肝癌高发区饮用塘水对肝细胞ＤＮＡ修复合成的影响，以探讨塘水的基因毒性。结果：①该地区饮用塘水浓缩物对大鼠原代肝细胞ＵＤＳ试验呈阳性反应，且在一定剂量范围内（０．１～０．５ｍｇ／ｍｌ）显示良好的剂量─—效应关系；②塘水浓缩物剂量较高（０．５ｍｇ／ｍｌ）时，其ＵＤＳ试验ｃｐｍ值与强致癌物ＡＦＢ＿１（０．０μｇ／ｍｌ）组相近；③当塘水浓缩物剂量高达１．０ｍｇ／ｍｌ时，对大鼠肝细胞尚可产生细胞毒作用。提示该地区居民饮用塘水中可能存在基因毒性致癌物。
The rat primary hepatocyte UDS assay was used to study the effect of drinking pond water in a high incidence area of liver cancer on DNA synthesis and repair in hepatocytes to explore the genotoxicity of pond water. Results: (1) The drinking pond water concentrate in this area showed a positive response to the UDS test of rat primary hepatocytes, and showed a good dose-response relationship within a certain dose range (0.1-0.5 mg / ml). When the concentration of pond water concentrate is higher (0.5mg / ml), the cpm value of UDS test is similar to the group of strong carcinogens AFB_1 (0.0μg / ml); ③ When the concentration of pond water concentrate is as high as 1.0mg / ml , Can still produce cytotoxic effects on rat liver cells. Prompt residents in the region drinking pond water may be genotoxic carcinogens.