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目的 :探讨白细胞介素 10和干扰素γ在病毒性肝炎的诊断、治疗和预后判断等方面的意义。方法 :采用植物血凝素(PHA)刺激 89例慢性乙型肝炎患者外周血单个核细胞 (PBMC)分泌 IL- 10和 IFN- γ,采用 EL ISA法检测其水平。结果 :随着患者病情的加重 ,IL- 10和 IFN- γ水平均降低 ,且与肝脏损伤程度有密切关系。结论 :IL- 10和 IFN- γ参与了肝炎的病理过程。当两者产生水平低于正常水平近一半时 ,预示肝脏受损较重 ,病情较重 ,预后较差。
Objective: To investigate the significance of interleukin-10 and interferon-γ in the diagnosis, treatment and prognosis of viral hepatitis. Methods: Peripheral blood mononuclear cells (PBMCs) from 89 patients with chronic hepatitis B were stimulated with phytohemagglutinin (PHA) to secrete IL-10 and IFN-γ. ELISA was used to detect the levels of IL-10 and IFN-γ. Results: As the patient’s condition aggravated, the levels of IL-10 and IFN-γ decreased, and were closely related to the degree of liver injury. Conclusion: IL-10 and IFN-γ are involved in the pathological process of hepatitis. When the level of the two is lower than the normal level of nearly half, indicating a heavier liver damage, severe condition, the prognosis is poor.