RAPD tagging of a salt tolerant gene in rice

来源 :Chinese Science Bulletin | 被引量 : 0次 | 上传用户:sqe622
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A salt tolerant rice mutant was obtained through tissue culture. The inheritance of salt tolerance in F2 population of mutant crossed with its original variety under salt stress was studied. According to the standard, the ratio of salt tolerant: sensitive was about 3:1 suggesting that there was a major gene related to salt tolerance in the mutant. By RAPD analysis with 220 10_mer primers, the gene was tagged by a 1.0 kb DNA fragment named OPS12_10 which was located on chromosome 7. The genetic distance between the major salt tolerant gene was 16.4 cm. A salt tolerant rice mutant was obtained through tissue culture. The inheritance of salt tolerance in F2 population of mutant crossed with its original original variety under salt stress was studied. According to the standard, the ratio of salt tolerant: sensitive was about 3: 1 suggesting that there was a major gene related to salt tolerance in the mutant. By RAPD analysis with 220 10_mer primers, the gene was tagged by a 1.0 kb DNA fragment named OPS12_10 which was located on chromosome 7. The genetic distance between the major salt tolerant gene was 16.4 cm.
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