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建立了2,4-二硝基苯肼柱前衍生反相高效液相色谱紫外检测法测定血清中克拉霉素的含量,将在碱性条件下甲基叔丁基醚的血清萃取物,与2,4-二硝基苯肼在55℃酸性条件下反应30min,然后用乙腈-0.05mol/L pH7.2磷酸盐缓冲液(48∶52)在Alltima C18色谱柱上进行分离,在340nm检测衍生物。方法的线性范围为0.05~3.2mg/L(r=0.9993);检出限为30μg/L,绝对回收率大于89%,相对标准差小于10%。本方法已用于克拉霉素在健康受试者中的药代动力学研究。
The determination of clarithromycin in serum by 2,4-dinitrophenylhydrazine pre-column derivatization reversed-phase high performance liquid chromatography with ultraviolet detection was carried out. The serum extracts of methyl tert-butyl ether The 2,4-dinitrophenylhydrazine was reacted under acidic conditions at 55 ° C for 30 min, then separated on an Alltima C18 column with acetonitrile-0.05 mol / L pH 7.2 phosphate buffer (48:52) and detected at 340 nm derivative. The linear range of the method was 0.05-3.2 mg / L (r = 0.9993). The detection limit was 30 μg / L, the absolute recovery was more than 89% and the relative standard deviation was less than 10%. This method has been used for pharmacokinetic studies of clarithromycin in healthy subjects.