论文部分内容阅读
目的观察全身热应激预处理对提高小鼠抗缺氧能力的作用及探讨其可能机制。方法通过传统的空白对照实验:即74只雄性小鼠随机分为两组,实验组37只,对照组37只。实验组为热应激预处理(当小鼠肛温达到40~41℃后维持该温度区间10 min)+抗缺氧实验[在缺氧瓶中加入钠石灰(铺满瓶底即可),将实验组小鼠放入缺氧瓶中,盖紧瓶塞];对照组为不做任何预处理+抗缺氧实验。记录比较两组小鼠在缺氧环境中的存活时间。实验结果采用SPSS 10.0统计软件进行分析比较显著性。结果实验组小鼠存活时间为(28.46±7.60)min;对照组存活时间为(20.03±4.77)min,P<0.001,实验组小鼠存活时间比对照组长。结论热应激预处理对提高小鼠抗缺氧能力有一定作用,可能与热应激蛋白清除氧自由基减少氧自由基对细胞损伤有关。
Objective To observe the effect of systemic heat stress preconditioning on enhancing anti-anoxia in mice and to explore its possible mechanism. Methods The traditional blank control experiment: 74 male mice were randomly divided into two groups, 37 in the experimental group and 37 in the control group. Experimental group for heat stress pretreatment (when the mouse rectal temperature reached 40 ~ 41 ℃ to maintain the temperature range of 10 min) + anti-hypoxia experiment [sodium hypoglycemia in the oxygen bottle (covered bottle can be) The experimental group of mice into hypoxia bottle, cap tightly cork]; control group without any pretreatment + anti-hypoxia experiments. Record the survival time of the two groups of mice in hypoxic environment. The experimental results using SPSS 10.0 statistical software to analyze the significance of comparison. Results The survival time of mice in the experimental group was (28.46 ± 7.60) min and that in the control group was (20.03 ± 4.77) min, P <0.001. The mice in the experimental group survived longer than the control group. Conclusion Heat stress preconditioning may play an important role in enhancing the anti-hypoxic ability of mice, which may be related to the heat shock protein scavenging oxygen free radicals to reduce oxygen free radicals on cell injury.