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本实验采用鲎试验基质显色法检测D-氨基半乳糖(GaLN)形成急性肝衰竭(FHF)模型大鼠血浆内毒素(LPS)水平,并探讨了肝细胞生长素(HGF)对其影响。结果表明:FHF早期血浆LPS水平较正常对照组水平(49±6.94pg/ml)明显升高,且其水平随时间延长而自然降低。动态观察第9小时为高峰期(234±83.8pg/ml),30小时为最低水平(63.1±18.4pg/ml),与正常对照组比较分别具非常显著(P<0.01)或显著性差异(P<0.05)。结合肝组织病理学检查结果提示,内毒素血症出现在形成明显的肝损害之前。以HGF早期一次性腹腔注射治疗,发现9小时治疗组血浆LPS水平(149±41.9pg/ml)明显低于盐水对照组水平(234±83.8pg/ml,P<0.005)。相反,24小时HGF组LPS水平升高(266±55.9pg/ml)而明显高于盐水对照组水平(153±71.9pg/ml,P<0.001)。改变给药方式,则24小时两组LPS水平比较无显著性差异(115±14.5pg/ml与117±12.1pg/ml,P>0.05)。本研究进一步说明HGF、内毒素血症及肝损伤三者间紧密联系,并提示HGF治疗FHF所出现的内毒素血症以间歇或持续用药为宜。
In this study, the plasma endotoxin (LPS) level in rats with acute hepatic failure (FHF) induced by D-galactosamine (GaLN) was measured by 鲎 test substrate colorimetric method and the effect of hepatocyte growth-promoting factor (HGF) was also investigated. The results showed that the level of LPS in early FHF plasma was significantly higher than that in normal control group (49 ± 6.94pg / ml), and the level of LPS naturally decreased with time. The peak value of dynamic observation at the 9th hour was 234 ± 83.8pg / ml, the lowest was 30 hours (63.1 ± 18.4pg / ml), which was significantly (P <0.01) or significant difference compared with the normal control group P <0.05). Combined with liver histopathological examination results suggest that endotoxemia occurs before the formation of significant liver damage. In the early intraperitoneal injection of HGF, the level of plasma LPS in the 9-hour treatment group (149 ± 41.9 pg / ml) was significantly lower than that in the saline control group (234 ± 83.8 pg / ml, P <0.005). In contrast, LPS levels in the 24-h HGF group were significantly higher (266 ± 55.9 pg / ml) than those in the saline control group (153 ± 71.9 pg / ml, P <0.001). (P <0.05). There was no significant difference in LPS levels between the two groups at 24 hours (115 ± 14.5pg / ml vs 117 ± 12.1pg / ml, P> 0.05). This study further illustrates the close relationship between HGF, endotoxemia and liver injury, and prompts HGF treatment of endotoxemia occurred FHF intermittent or continuous medication is appropriate.