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目的探讨6-姜酚对丙酮醛诱导的HK-2肾小管上皮细胞(HK-2细胞)损伤的保护作用。方法采用丙酮醛诱导的HK-2细胞构建细胞损伤模型,并用6-姜酚进行干预。应用CCK-8法检测HK-2细胞的存活率;DAPI染色法检测HK-2细胞凋亡形态学变化。Western blot法检测活化的caspase-3蛋白表达。结果与对照组相比,模型组细胞存活率下降,差异有统计学意义(P<0.05),并出现明显凋亡特征;而加入6-姜酚处理后,细胞存活率及活化的caspase-3蛋白水平降低,差异有统计学意义(P<0.05)。结论 6-姜酚对丙酮醛诱导的HK-2细胞损伤具有保护作用。
Objective To investigate the protective effect of 6-gingerol on pyruvate-induced injury of HK-2 renal tubular epithelial cells (HK-2 cells). Methods Pyruvate-induced HK-2 cells were used to establish cell injury models and treated with 6-gingerol. The viability of HK-2 cells was detected by CCK-8 assay. The morphological changes of HK-2 cells were detected by DAPI staining. The expression of activated caspase-3 protein was detected by Western blot. Results Compared with the control group, the survival rate of the model group decreased significantly (P <0.05), and the obvious apoptotic characteristics were observed. However, the cell viability and activation of caspase-3 The protein level decreased, the difference was statistically significant (P <0.05). Conclusion 6-Gingerol has a protective effect on pyruvate-induced injury of HK-2 cells.