【摘 要】
：
DNA fragments obtained from Sau3AI partially digested total DNA of Bacillus pumilus UN31-C-42 are first inserted into BamHI site of pSUPV4, a promoter-probe vec
【机 构】
：
Sichuan Key Laboratory of Molecular Biology and Biotechnology
论文部分内容阅读
DNA fragments obtained from Sau3AI partially digested total DNA of Bacillus pumilus UN31-C-42 are first inserted into BamHI site of pSUPV4, a promoter-probe vector. The recombinant DNA molecules are transformed into Escherichia coli cells and eight-three Kanr clones (named pSUBp1- pSUBp83) are obtained. The inserted fragments in pSUBp53, pSUBp57, pSUBp21, which showed high level of kanamycin - resistance, are sequenced and analyzed, respectively. These fragments contain some conserved sequences of prokaryotic gene promoters, such as TATAAT and TTGACA box. The promoter fragment Bp53 could efficiently promote the alkaline protease gene of B.pumilus expression not only in E.coli but also in B.subtilis cells.
其他文献
A novel β-cyclodextrin derivative, L-cystine bridged bis(β-cyclodextrin) 1 has been synthesized and characterized by 1H NMR, 13C NMR, IR, Raman and combustion
Thin films of hydrogenated amorphous germanium (a-Ge:H) were prepared by radio frequency glow discharge deposition at various substrate temperatures. The hydrog
This paper proposes a video watermarking technology to hide copyright information by a slight modification of the motion vector in MPEG-2 video bitstream. In th
The paper introduces the -35 kV/200 kW high voltage power supply (HVPS) [1]which is specially used to test klystron units in LHCD [2] system. The new klystrons
以CdZnTe核探测器的工作原理为依据,探测器内反应的随机性和反应产生的电子空穴对数目的统计规律为物理模型,应用Visual C++自行编制了蒙特卡罗模拟软件.模拟了γ射线在CdZnT
近日,一段梅赛德斯-奔驰全新M级越野车的广告在网络上点击率飙升,费德勒驾驶着一辆梅赛德斯-奔驰ML350,从白雪皑皑的苏黎世闪到了酷热的美国红石谷,一路穿越大雨倾盆的卡梅尔
生命中总有那么一个夜晚,我们不是为了等待结束,而是为了永远铭记。3月28日夜,北京男篮力压“七冠王”广东男篮,赢得关键性的总决赛赛点,随后一举夺得队史上首座CBA冠军奖杯
The adhesion of soil to solid surface is a complicated interfacial reaction that relates to many factors. Quantitative descriptions of the forming procedures an
从素有“东方巴黎”的上海,到誉为“人间天堂”的苏州,整整一年过去了,东风悦达起亚K5迎来了华丽的转身,一年间,K5究竟经历了如何的变化?一场换“芯”行动,正在悄然而起。
F
We extend the classical compound Poisson risk model to the case where the premium income process, based on a Poisson process, is no longer a linear function.For