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作者报告了以产肠毒素性大肠杆菌不耐热肠毒素(LT)为粘膜佐剂,与全流感病毒灭活疫苗同时口服免疫小鼠的效果.甲型流感病毒X-31株经培养浓缩并以蔗糖梯度离心后,稀释成1mg/ml浓度,按0.025%浓度加入福马林于4C作用3天使病毒完全灭活;采用6～10周龄BALB/c雌性小鼠于轻度麻醉下口饲不同剂量纯化的灭活X-31病毒(加或不加25μgLT),间隔1周免疫3次.同时采用单剂腹腔注射(不加LT)或末次口服后1周腹腔注射加强免疫1剂(不加LT)作为对照.末次接种后3周采集小鼠血清及肺与鼻腔冲洗液标本,经霍乱弧菌受体破坏酶处理后,用ELISA检测样品的抗病毒IgG和IgA.以标准方法测定血凝抑制抗体及中和抗体滴度;在接种后3周或
The authors reported the effect of oral immunization of mice with enterotoxigenic Escherichia coli heat-labile enterotoxin (LT) as a mucosal adjuvant in combination with a full-blown virus inactivated vaccine. The influenza A virus X-31 strain was concentrated and cultured After sucrose gradient centrifugation, diluted to a concentration of 1 mg / ml, 0.025% of the concentration was added to doxorubicin at 4C for 3 days to completely inactivate the virus; BALB / c female mice aged 6 to 10 weeks were dosed under mild anesthesia Immunization with different doses of purified inactivated X-31 virus (with or without 25 μg of LT) was given 3 times at 1 week intervals with 1 dose boosted with either single intraperitoneal (without LT) or intraperitoneal injection 1 week after last oral dose Plus LT) as control.After 3 weeks of the last vaccination, mice serum and lung and nasal wash samples were collected and tested for anti-viral IgG and IgA by ELISA after Vibrio cholerae receptor-damaging enzyme treatment.According to standard method Inhibition of antibody and neutralizing antibody titer; 3 weeks after inoculation or