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目的探讨子宫内膜异位症及非子宫内膜异位症患者卵泡液对精子与透明带结合能力的影响。方法卵泡液取自２０例进行体外授精胚胎移殖的不孕症患者，其中１０例为子宫内膜异位症，１０例为单纯炎症性输卵管不通。应用半透明带配对研究、培养液ＥＢＳＳ／ＢＳＡ做为对照，观察子宫内膜异位症及非子宫内膜异位症患者卵泡液对精子与透明带结合能力的影响。结果经子宫内膜异位症患者卵泡液与非子宫内膜异位症患者卵泡液处理后的精子与透明带结合的数目均明显低于经培养液处理后的精子与透明带结合的数目（９１±２１与２３９±３５，Ｐ＜０．００１；１０９±２２与２１１±４２，Ｐ＜０．００５），经子宫内膜异位症患者卵泡液处理的精子结合半透明带指数明显低于经非子宫内膜异位症患者卵泡液处理后的精子结合半透明带指数（３６±４与５４±４，Ｐ＜０．００５）。结论子宫内膜异位症患者卵泡液对精子与透明带结合能力具有较强的抑制作用，子宫内膜异位症患者卵泡液对配子相互作用的影响可能是其不孕的原因之一。
Objective To investigate the effect of follicular fluid on the binding ability between sperm and zona pellucida in endometriosis and non-endometriosis patients. Methods Follicular fluid was obtained from 20 infertile patients who underwent in vitro fertilization embryo transfer, of which 10 were endometriosis and 10 were simple inflammatory tubal occlusion. The translucent band matching study and culture fluid EBSS / BSA were used as controls to observe the effect of follicular fluid on the binding capacity of sperm and zona pellucida in patients with endometriosis and non-endometriosis. Results The number of follicular fluid-treated sperm and zona pellucida in follicular fluid and non-endometriosis patients after endometriosis patients were significantly lower than the number of combined sperm and zona pellucida treated by culture fluid 91 ± 21 and 239 ± 35, P <0.001; 109 ± 22 and 211 ± 42, P <0.005). The index of sperm-binding translucent zone in the follicular fluid of patients with endometriosis was significantly lower than that of the control The sperm-bound translucent zone index (36 ± 4 and 54 ± 4, P <0.005) was associated with follicular fluid in non-endometriosis patients. Conclusions Follicular fluid in patients with endometriosis has a strong inhibitory effect on the binding capacity of sperm and zona pellucida. The effect of follicular fluid on the interaction of gametes in endometriosis patients may be one of the reasons for its infertility.