论文部分内容阅读
目的 探讨人胚中脑腹侧神经干细胞的分离、增殖条件及分化方向。方法 应用表皮生长因子 (ＥＧＦ)和成纤维细胞生长因子 2 (ＦＧＦ 2 )使人胚中脑腹侧幼稚细胞维持在未分化状态并促进其增殖 ;通过克隆分析、细胞增殖曲线及流式细胞仪检测培养细胞的增殖能力 ;应用免疫组织化学
Objective To investigate the isolation, proliferation and differentiation of human neural stem cells in the ventral midbrain of human embryo. Methods Epidermal growth factor (EGF) and fibroblast growth factor 2 (FGF 2) were used to maintain the ventral mesenchymal cells of human embryo in an undifferentiated state and to promote their proliferation. Through clonal analysis, cell proliferation curve and flow cytometry Detection of proliferation of cultured cells; the application of immunohistochemistry