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Objective:To develop a novel artificial antigen-presenting system for efficiently inducing melanoma-specific CD8~+CD28~+ cytotoxic T lymphocyte(CTL) responses.Methods:Cell-sized Dynabeads? M-450 Epoxy beads coated with H-2K~b:Ig-TRP2_(1811_11K_ and anti-CD28 antibody were used as artificial antigen-presenting cells(aAPCs) lo induce melanoma-specific CD8*CD28’ CTL responses with the help of IL-2I and IL-I5.Dimer staining,proliferation,ELISPOT,and cytotoxicity experiments were conducted to evaluate the frequency and activity of induced CTLs.Results:Dimer staining demonstrated that the new artificial antigen-presenting system efficiently induced melanoma TRP2-specific CD8CD28“ CTLs.Proliferation and ELISPOT assays indicated that the induced CTLs rapidly proliferate and produce increased IFN- y under the slimulalion of H-2K:Ig-TRP2-aAPCs,TL-15,and IL-21.In addition,cytoloxicily experiments showed lhat induced CTLs have specific killing activity of target cells.Conclusions:The new artificial antigen-presenting system including aAPCs plus IL-21 and IL-15 can induce a large number of antigen-specific CD8~+CD28~+ CTLs against the melanoma.Our study provides evidence for a novel adoptive immunotherapy against tumors.
Objective: To develop a novel artificial antigen-presenting system for efficiently inducing melanoma-specific CD8 ~ + CD28 ~ + cytotoxic T lymphocyte (CTL) responses. Methods: Cell-sized Dynabeads® M-450 Epoxy beads coated with H-2K ~ b : Ig-TRP2_ (1811_11K_ and anti-CD28 antibody were used as artificial antigen-presenting cells (aAPCs) lo induce melanoma-specific CD8 * CD28 ’CTL responses with the help of IL-2I and IL-I5.Dimer staining, proliferation, ELISPOT, and cytotoxicity experiments were conducted to evaluate the frequency and activity of induced CTLs. Results: Dimer staining of that the new artificial antigen-presenting system efficiently induced melanoma TRP2-specific CD8 CD28 ”" CTLs.Proliferation and ELISPOT assays indicated that the induced CTLs Rapid proliferate and produce increased IFN-y under the slim tumor of H-2K: Ig-TRP2-aAPCs, TL-15, and IL- 21.In addition, cytoloxicily experiments showed lhat induced CTLs have specific killing activity of target cells. The new artifici al antigen-presenting system including aAPCs plus IL-21 and IL-15 can induce a large number of antigen-specific CD8 ~ + CD28 ~ + CTLs against the melanoma. Our study provides evidence for a novel adoptive immunotherapy against tumors.