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目的检测血细胞减少患者外周血红细胞和中性粒细胞细胞膜糖基磷脂酰肌醇(GPI)连接的补体调节蛋白衰变加速因子(CD55)和膜反应性溶血抑制物(CD59)表达情况,并探讨其临床意义。方法 2006年7月-2011年3月,采用直接免疫荧光标记法流式细胞仪检测182例血细胞减少患者外周血CD55及CD59表达情况,其中阵发性睡眠性血红蛋白尿(PNH)9例,再生障碍性贫血(AA)-PNH综合征8例,AA 83例,骨髓增生异常综合征51例,自身免疫性溶血性贫血11例,造血功能停滞6例,缺铁性贫血7例,巨幼细胞性贫血4例,脾功能亢进3例。结果 PNH及AA-PNH患者CD55、CD59抗原缺失率均较其他血细胞减少者明显增高。结论流式细胞仪检测外周血中红细胞和中性粒细胞膜CD55和CD59抗原表达缺失率是目前诊断PNH可靠和敏感的方法,也是对PNH、AA-PNH早期诊断敏感指标,并且PNH克隆检测还能为诊断疾病提供鉴别诊断依据。
Objective To investigate the expression of complement regulatory protein decay accelerating factor (CD55) and membrane reactive hemolytic inhibitor (CD59) of glycosylphosphatidylinositol (GPI) in peripheral blood erythrocytes and neutrophils in patients with cytopenia Clinical significance. Methods From July 2006 to March 2011, the expression of CD55 and CD59 in peripheral blood of 182 patients with cytopenia were detected by direct immunofluorescence labeling, including 9 cases of paroxysmal nocturnal hemoglobinuria (PNH) Eight cases of AA, PNH, 83 cases of AA, 51 cases of myelodysplastic syndrome, 11 cases of autoimmune hemolytic anemia, 6 cases of hematopoietic arrest, 7 cases of iron deficiency anemia, 4 cases of anemia, 3 cases of hypersplenism. Results The deletion rate of CD55 and CD59 in patients with PNH and AA-PNH were significantly higher than those in other cytopenias. Conclusion Flow cytometry is a reliable and sensitive method for the diagnosis of PNH and is also a sensitive indicator for the early diagnosis of PNH and AA-PNH. The detection of PNH clone can also detect the loss of CD55 and CD59 antigen expression in peripheral blood of erythrocytes and neutrophils. To provide diagnostic evidence for the diagnosis of disease basis.