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背景:血管内皮生长因子受体对许多实体瘤的血管生成和预后起着重要作用,但血管内皮生长因子受体与肺癌预后的关系研究报道较少。目的:探讨肺癌中血管内皮生长因子受体Flt1和KDR的表达及其与转移及预后的关系。设计:以病理标本为研究对象单一样本研究。单位:一所大学的病理学教研室。材料:随机选取青岛大学医学院附属医院胸外科1985-01/1990-12经手术切除的75个肺癌标本。2002-09/11月间在青岛大学医学院病理学教研室进行实验。方法:应用免疫组织化学PowerVisionTM-9000(PV-9000)法,测定75个肺癌标本中Flt1和KDR的表达。主要观察指标:肺癌中血管内皮生长因子受体Flt1和KDR的表达及其与年龄、性别、病理类型、组织学分级、肿瘤大小、淋巴结转移和预后的关系。肿瘤细胞、纤维母细胞中Flt1和KDR表达的关系。结果:肺癌组织中Flt1和KDR的表达较为广泛,主要位于肿瘤细胞胞浆及胞膜上,纤维母细胞和血管内皮细胞胞浆中亦有表达。Flt1和KDR在肿瘤细胞中的阳性率均显著高于在间质纤维母细胞中的表达(χ2=6.07,5.88,P<0.05)。肿瘤细胞及纤维母细胞中该两种受体的阳性率在不同年龄、不同性别及不同病理类型、不同病理分级之间差异均无显著性意义(P>0.05)。肿瘤细胞中Flt1和KDR的阳性表达率在3组不同大小的肿瘤间差异?
BACKGROUND: Vascular endothelial growth factor receptors play an important role in the angiogenesis and prognosis of many solid tumors. However, there are few reports on the relationship between the receptors of vascular endothelial growth factor and the prognosis of lung cancer. Objective: To investigate the expression of vascular endothelial growth factor receptor Flt1 and KDR in lung cancer and its relationship with metastasis and prognosis. Design: a single sample of the pathological specimens for the study. Unit: a University Department of Pathology. MATERIALS: Seventy-five lung cancer specimens surgically excised from January 1985 to December 1990 were randomly selected from the Department of Thoracic Surgery, Affiliated Hospital of Medical College of Qingdao University. 2002 - September / November in Qingdao University School of Medicine Pathology Department for experiments. Methods: Immunohistochemistry PowerVisionTM-9000 (PV-9000) method was used to determine the expression of Flt1 and KDR in 75 lung cancer specimens. MAIN OUTCOME MEASURES: The expression of Flt1 and KDR in lung cancer and their relationship with age, sex, pathological type, histological grade, tumor size, lymph node metastasis and prognosis. Tumor cells, fibroblasts Flt1 and KDR expression. Results: The expressions of Flt1 and KDR in lung cancer tissues were more extensive, mainly located in the cytoplasm and membrane of tumor cells, and were also expressed in the cytoplasm of fibroblasts and vascular endothelial cells. The positive rates of Flt1 and KDR in tumor cells were significantly higher than those in interstitial fibroblasts (χ2 = 6.07,5.88, P <0.05). The positive rates of these two receptors in tumor cells and fibroblasts were not significantly different between different age groups, different genders, different pathological types and pathological grades (P> 0.05). The positive rates of Flt1 and KDR in tumor cells were different among the three tumors of different sizes.