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目的:观察肝素与23种染料之间的相互作用。方法:利用淀粉琼脂糖凝胶电泳及聚丙烯酰胺凝胶电泳,观察染料加肝素后电泳行为的变化。结果:淀粉琼脂糖凝胶电泳中染料加肝素后多数结果是泳速变慢,有一些留在原点,也有前移、后退者。二氯荧光素、荧光红、荧光素、甲酚红、苯胺蓝、溴酚蓝、茜素红S、胭脂红、亮绿、氨基黑10B、丽春红G、曙红、苯胺黑、氯酚红加肝素后泳速变慢。亚甲蓝、灿烂甲酚蓝、甲基紫加肝素后电泳留在原点。刚果红、洋红加肝素后电泳变化不明显。四氯荧光素、氯化硝基四氮唑蓝加肝素后电泳荧光看不清。聚丙烯酰胺凝胶电泳中溴酚蓝加入肝素后泳速明显变慢,随着肝素量增加溴酚兰的泳速加快。与淀粉琼脂糖凝胶电泳结果一致,但它的电泳结果更明显。结论:本次所研究的23种染料,几乎都能与肝素发生相互作用,给它们的进一步深入研究打下基础。
Objective: To observe the interaction between heparin and 23 dyes. Methods: Starch agarose gel electrophoresis and polyacrylamide gel electrophoresis were used to observe the electrophoresis behavior of the dye and heparin. Results: Most of the results of starch agarose gel electrophoresis dye and heparin is swimming speed is slow, some stay in the origin, but also forward, backward. Fluorescein, cresol red, aniline blue, bromophenol blue, alizarin red S, carmine, brilliant green, amino black 10B, Ponceau G, eosin, aniline black, After red plus heparin, the swimming speed is slower. Methylene blue, brilliant cresyl blue, methyl purple plus heparin electrophoresis stay at the origin. Congo red, magenta plus heparin electrophoresis change was not obvious. Fluorescent tetrachlorofluorescein, nitrochlorothiazide blue plus heparin electrophoresis fluorescence can not see clearly. The polyacrylamide gel electrophoresis of bromophenol blue after the addition of heparin significantly slower swimming speed, with increasing the amount of bromophenol blue swimming faster. Consistent with the results of starch agarose gel electrophoresis, but its electrophoresis results more pronounced. Conclusion: The 23 kinds of dyes studied in this article almost all can interact with heparin, laying a foundation for their further study.