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目的:鉴定高脂培养对肝星形细胞活化的影响。方法:培养HSC-T6细胞系,加入含有游离脂肪酸的高脂培养基处理,利用LPS处理活化,通过检测α-SMA的表达分析星形细胞的活化程度,通过Q-PCR分析HSCs细胞胶原的表达,通过Q-PCR实验分析LPS相关通路靶基因表达情况情况。结果:高脂培养能够抑制LPS诱导的HSC-T6细胞增殖,降低HSC-T6细胞α-SMA和胶原I和TIMP-1表达的水平,Q-PCR的分析表明,高脂培养能够抑制HSCs活化后的NF-κB通路下游靶基因MCP-1和IL-6的表达。结论:在体外培养实验中,高脂培养能够抑制LPS诱导的HSC-T6细胞活化。
Objective: To identify the effects of high-fat culture on the activation of hepatic stellate cells. Methods: The HSC-T6 cell line was cultured and treated with high-fat medium containing free fatty acids, activated by LPS, the activation of astrocytes was analyzed by detecting the expression of α-SMA, and the expression of collagen in HSCs was analyzed by Q-PCR , Through Q-PCR experiments analysis LPS related pathway target gene expression situation. Results: High-fat culture could inhibit the proliferation of HSC-T6 cells induced by LPS and decrease the expression of α-SMA and collagen I and TIMP-1 in HSC-T6 cells. Analysis by Q-PCR showed that HSF could inhibit the activation of HSCs NF-|ÊB pathway downstream target gene MCP-1 and IL-6 expression. Conclusion: In vitro culture experiments, high-fat culture can inhibit LPS-induced HSC-T6 cell activation.