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本研究选用抗性相对较弱的水稻 (Oryza sativa L.)品种越富为实验材料 ,利用 DDRT- PCR(Differential Display Reverse Transcription- PCR)技术研究了在 4～ 6℃的低温胁迫下 Isoprothiolane(IPT)诱导水稻幼苗抗性增强的部分分子机理。结果表明 :在正常生长条件下 ,IPT处理对水稻秧苗根的影响与对照基本相同 ;但在低温胁迫下 ,两种处理的水稻幼苗根中 m RNA存在明显差异。这些差异基因有的是在低温胁迫下由 IPT诱异表达的 ;还有一些基因是在低温胁迫下对照中没有而 IPT处理下仍然有表达的。经二次扩增及反向 Northern确定了 5个差异表达的基因
In this study, the richer rice varieties (Oryza sativa L.) with relatively weaker resistance were used as experimental materials. The effects of Isoprothiolane (IPT) under low temperature stress of 4 ~ 6 ℃ were studied by DDRT-PCR (Differential Display Reverse Transcription- ) Induced partial resistance of rice seedlings to some molecular mechanisms. The results showed that under the normal growth conditions, the effect of IPT on the seedling roots of rice was basically the same as that of the control. However, under the low temperature stress, there was a significant difference in m RNA among the two treatments. Some of these differentially expressed genes were induced by IPT under low temperature stress; others were still expressed under the condition of low temperature stress but not under the control of IPT. Five differentially expressed genes were identified by secondary amplification and reverse Northern analysis