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目的 :观察大肠腺瘤和腺癌组织中 PTEN、缺氧诱导因子 - 1α(HIF- 1α)表达情况 ,探讨两者在大肠腺瘤一腺癌序列发生发展中的作用及相互关系。方法 :采用原位杂交技术检测 18例大肠腺瘤和 6 2例大肠腺癌组织中 PTEN- m RNA、HIF-1αm RNA,用免疫组织化学 S- P方法检测 VEGF蛋白的表达。比较三者与大肠癌 Dukes分期、淋巴结转移、肝转移及组织学分级等病理特征的关系。结果 :18例大肠腺瘤 PTEN- m RNA、HIF- 1αm RNA、VEGF蛋白阳性表达率分别为 77.8% (14 / 18) ,38.9% (7/ 18) ,33.3% (6 / 18) ,大肠腺癌分别为 5 1.6 % (32 / 6 2 ) ,6 7.7% (42 / 6 2 ) ,5 9.7% (37/ 6 2 )。腺瘤组织 HIF- 1αm RNA、VEGF阳性表达率均低于腺癌细织 (P <0 .0 5 ) ,腺瘤组织 PTEN- m RNA阳性表达率显著高于腺癌组织 (P <0 .0 5 )。大肠腺癌细织中 PTEN- m RNA吸光值随 Dukes分期增高而逐渐降低 ,A期 (0 .1782± 0 .0 2 71) ,B期 (0 .15 38± 0 .0 397)分别与 C+D期(0 .14 70± 0 .0 5 2 4 )比较差异均具有显著性 (P <0 .0 5 )。 HIF- 1αm RNA、VEGF蛋白吸光值随 Dukes分期增高而逐渐升高 ,A期 ,HIF- 1α(0 .10 2 9± 0 .0 4 5 ) ,VEGF(0 .12 0 7± 0 .0 4 36 )分别与 B期 (0 .16 5 6± 0 .0 32 9、0 .15 72± 0 .0 5 1
Objective: To observe the expression of PTEN and hypoxia inducible factor - 1α (HIF - 1α) in colorectal adenocarcinoma and adenocarcinoma, and to explore their roles in the development of colorectal adenoma and adenocarcinoma. Methods: The expressions of PTEN-m RNA and HIF-1αmRNA in 18 cases of colorectal adenoma and 62 cases of adenocarcinoma of the colon were detected by in situ hybridization. The expression of VEGF protein was detected by immunohistochemical S-P method. To compare the three with Dukes staging of colorectal cancer, lymph node metastasis, liver metastasis and histological grade and other pathological features. Results: The positive rates of PTEN-m RNA, HIF-1αmRNA and VEGF in 18 cases of colorectal adenomas were 77.8% (14/18), 38.9% (7/18), 33.3% (6/18) Cancer were 5 1.6% (32/62), 6 7.7% (42/62) and 57.7% (37/62), respectively. The positive rate of HIF-1αmRNA and VEGF in adenoma was lower than that in adenocarcinoma (P <0.05), and the positive rate of PTEN-m RNA in adenoma was significantly higher than that in adenocarcinoma (P <0. 0 5). The absorbency of PTEN-m RNA in the tissue of colorectal adenocarcinoma decreased gradually with the increase of Dukes staging. The A phase (0.1782 ± 0.0221) and B (0.1538 ± 0.0397) + D (0.14 70 ± 0 .0 5 2 4) were significant differences (P <0. 05). The absorbance of HIF-1αmRNA and VEGF protein gradually increased with the increase of Dukes staging. The expression of HIF-1α (0.1229 ± 0.045), VEGF (0.1270 ± 0.400) 36) and B (0.165 6 ± 0 .032 9,0 .15 72 ± 0 .0 5 1 respectively