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为了探讨氟对体外骨器官培养长骨细胞周期分布和细胞凋亡的影响，我们采用体外骨器官培养的方法．流式细胞术检测长骨细胞周期分布、ＤＮＡ含量和细胞凋亡。结果发现１．染氟２．５～５.０μｇ／ｍＬ，对体外培养长骨细胞ＤＮＡ含量和细胞周期分布影响不大。染氟１０．０μｇ／ｍＬ，Ｓ期细胞数增加，但Ｇ０／Ｇ１期和Ｇ２／Ｍ期细胞无明显改变。染氟２０．０μｇ／ｍＬ，Ｓ期细胞数增加，Ｇ2／Ｍ期细胞数减少，但Ｇ０／Ｇ１期细胞无明显改变。表明氟作为一种刺激因子，干扰了细胞周期正常的信号传导，使Ｓ期的细胞进入Ｇ２／Ｍ期受到抑制，细胞周期停滞在Ｓ期，从而使分裂期细胞减少，导致细胞增殖受到影响。２·染氟浓度在２·５～１０·００μｇ／ｍＬ范围，未见氟对体外培养长骨细胞凋亡有明显影响。氟２０．０μｇ／ｍＬ可诱导体外培养长骨细胞凋亡。结果表明·氟对骨骼的损伤与氟诱导细胞凋亡和骨细胞周期分布异常改变有密切关系。
In order to investigate the effect of fluoride on the periodontal distribution and apoptosis of cultured long bone cells in vitro, we used a method of in vitro bone organ culture. Flow cytometry was used to detect the long-term cell cycle distribution, DNA content and apoptosis. The results found 1. Fluorine 2.5 ~ 5.0μg / mL, in vitro cultured long bone cells DNA content and cell cycle distribution is not affected. With fluoride 10.0μg / mL, S phase cells increased, but the G0 / G1 phase and G2 / M phase cells did not change significantly. Fluorine 20.0μg / mL, the number of S phase cells increased, G2 / M phase cells decreased, but the G0 / G1 phase cells did not change significantly. As a stimulating factor, fluorine interferes with normal cell cycle signaling and inhibits S phase cells from entering the G2 / M phase and arrests the cell cycle in S phase, resulting in reduced cell division and consequent cell proliferation. 2. The concentration of fluoride in the range of 2.5 ~ 10.00μg / mL, no fluoride on cultured long bone cells significantly affect the apoptosis. Fluorine 20.0μg / mL can induce long bone cell apoptosis in vitro. The results show that fluoride has a close relationship with bone damage induced by fluoride and apoptosis induced by fluoride and the abnormal changes of cell cycle distribution.