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含油微藻的破碎是微藻制油过程中的重要环节。为此,利用清华大学自主研制的高压脉冲电源(THU-PEF4)系统,针对小球藻的光合活性和叶绿素质量浓度这2个生物量,重点考察了高压脉冲电场强度、脉冲宽度、脉冲重复频率、电场极性及样品电导率对小球藻处理效果的影响,在此基础上结合双荧光染色法和流式细胞仪研究了高压脉冲电场(PEF)对微藻细胞的穿孔破碎效果。研究发现高压脉冲电场强度和脉冲注入能量密度是影响高压脉冲电场处理效果的关键因素,而脉冲宽度、脉冲重复频率、电场极性对小球藻的处理效果影响不大。当电场强度从2.5 MV/m增加到5.0 MV/m时,20 m S/m电导率下的小球藻细胞破碎率从17.21%增加至83.29%;当脉冲注入能量密度从8.9 k J/L增加到149.52 k J/L时,4.5 MV/m电场强度作用下的小球藻细胞破碎率从9.78%提高到81.78%。
Broken oil microalgae is an important part of microalgae oil production process. Therefore, using the high voltage pulse power (THU-PEF4) system independently developed by Tsinghua University, we focused on the photosynthetic activity and chlorophyll concentration of Chlorella vulgaris and investigated the effects of high-voltage pulsed electric field, pulse width, pulse repetition frequency , The electric field polarity and the conductivity of the sample on the treatment of Chlorella were studied. Based on this, the effect of PEF on the micro-algae cell perforation and fragmentation was studied by dual-fluorescence staining and flow cytometry. The results show that the intensity of high-voltage pulse and the energy density of implanted pulse are the key factors affecting the effect of high-voltage pulsed electric field. However, pulse width, pulse repetition frequency and electric field polarity have little effect on the treatment effect of Chlorella. When the electric field intensity increased from 2.5 MV / m to 5.0 MV / m, the cell disruption rate of Chlorella increased from 17.21% to 83.29% under the conductivity of 20 m S / m. When the pulse energy density increased from 8.9 kJ / L When it increased to 149.52 kJ / L, the breaking rate of Chlorella cells was increased from 9.78% to 81.78% under the electric field of 4.5 MV / m.