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[目的]探讨大鼠肝纤维化形成过程中肝组织miR-21、miR-29b、TGF-β1、Smad3及Smad7表达水平的动态变化,研究miR-21、miR-29b与TGF-β1/Smad3、Smad7信号通路之间的关系。[方法]80只SD大鼠,随机分为2组,每组40只。其中模型组予以皮下注射60%CCl4,0.3ml/100g体重,每周2次,复制肝纤维化动物模型。于造模0、3、6、9、12周分别随机抽取8只大鼠处死,取肝组织行相关指标检测。采用qRT-PCR检测肝组织中miR-21、miR-29b及TGF-β1mRNA的相对表达量;Western blot检测肝组织中Smad3、Smad7的蛋白水平;日本MPIAS-500多媒体真彩色图像分析系统作肝内胶原定量分析。[结果]与正常组比较,随肝纤维化的形成,模型组在3、6、9、12周肝细胞内的miR-21、TGF-β1mRNA和Smad3的表达量逐渐增加;miR-29b和Smad7的表达量逐渐减少。且miR-21与Smad7呈负相关(r=-0.69,P=0.046),miR-29b与Smad3呈负相关(r=-0.53,P=0.037)。[结论]随着肝纤维化的形成,肝组织中miR-21、TGF-β1mRNA和Smad3的表达上调,miR-29b和Smad7的表达下调。miR-21与miR-29b可能分别参与调节Smad3、Smad7的表达,经TGF-β1/Smad3、Smad7信号通路共同参与肝纤维化的发生。
[Objective] To investigate the dynamic changes of liver tissue expression of miR-21, miR-29b, TGF-β1, Smad3 and Smad7 during the process of hepatic fibrosis in rats, Smad7 signaling pathway between the relationship. [Methods] Eighty SD rats were randomly divided into two groups of 40 rats. The model group was subcutaneously injected with 60% CCl4, 0.3ml / 100g body weight, twice a week, replicating liver fibrosis animal model. At 0, 3, 6, 9, and 12 weeks after operation, 8 rats were randomly selected and sacrificed. Relevant indexes of liver tissues were determined. The relative expression of miR-21, miR-29b and TGF-β1mRNA in liver tissues were detected by qRT-PCR. The protein levels of Smad3 and Smad7 in liver tissues were detected by Western blot. The MPIAS-500 multimedia true color image analysis system Quantitative analysis of collagen. [Results] Compared with the normal group, the expression of miR-21, TGF-β1mRNA and Smad3 in the hepatocytes of the model group increased gradually at 3, 6, 9 and 12 weeks with the formation of hepatic fibrosis; miR-29b and Smad7 The amount of expression gradually decreased. There was a negative correlation between miR-21 and Smad7 (r = -0.69, P = 0.046). MiR-29b was negatively correlated with Smad3 (r = -0.53, P = 0.037). [Conclusion] With the formation of hepatic fibrosis, the expression of miR-21, TGF-β1mRNA and Smad3 in liver tissue were upregulated, and the expressions of miR-29b and Smad7 were down-regulated. miR-21 and miR-29b may participate in the regulation of Smad3 and Smad7 expression respectively, and participate in the development of hepatic fibrosis via TGF-β1 / Smad3 and Smad7 signaling pathways.