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δ-氨基乙酰丙酸脱水酶(ALA-D)是催化二分子δ-氨基乙酰丙酸(ALA)合成卟胆元(PBG)的酶,大部分存在于红细胞中。测定红细胞ALA-D,对于估价职业性低浓度铅接触是一个可靠的指标。ALA-D测定通常采用Bonsignore等的方法,正常人血液在pH 6.4～6.8的范围内进行保温;然而,在职业性铅接触者中,测定ALA-D的最适pH值较正常人为低,即pH为6.0。作者在不同pH值(6.8及6.0)下进行保温分析ALA-D,其结果以活性及
Delta-aminolevulinic acid dehydratase (ALA-D) is an enzyme that catalyzes the synthesis of porphyrin (PBG) by two molecules of delta-aminolevulinic acid (ALA), most of which are present in erythrocytes. Determination of red blood cell ALA-D, for the valuation of occupational low concentrations of lead exposure is a reliable indicator. Normal ALA-D assay is based on the method of Bonsignore et al. Normal blood is incubated at pH 6.4-6.8. However, in the case of occupational lead exposure, the optimal ALA-D pH is lower than normal The pH is 6.0. The authors conducted thermal analysis of ALA-D at different pH values (6.8 and 6.0), the results of which were based on activity and