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作者研究了阿霉素磁性白蛋白微球在靶位及其它组织内的处置。制备将含有100mg磁铁矿粉末的25～35%W/W混悬液及200μl盐酸阿霉素溶液(50mg/ml)加至250μl牛血清白蛋白溶液(400mg/ml)中混匀,再加棉子油30ml,在4℃下用125W超声处理2分钟得到磁性乳液,将此磁乳以每分钟100±10滴的速度滴加至120±5℃的棉子油100 ml中,在1500rpm的转速下继续保温搅拌10分钟,再将此混合液迅速冷却至20℃,用无水醚洗涤4次,然后用倾泻法在300-G巴的外磁场作用下,将未乳化磁铁矿沉淀与阿霉素磁性白蛋白微球分离,最后将磁性白蛋白微球制
The authors studied the treatment of doxorubicin magnetic albumin microspheres at the target site and other tissues. Preparation A 25 to 35% W / W suspension containing 100 mg of magnetite powder and 200 μl of doxorubicin hydrochloride solution (50 mg / ml) were added to 250 μl of bovine serum albumin solution (400 mg / ml) Cottonseed oil 30ml, at 4 ° C with 125W sonicated for 2 minutes to obtain a magnetic emulsion, the magnetic emulsion at a rate of 100 ± 10 drops per minute was added dropwise to 120 ± 5 ℃ 100ml cottonseed oil, at 1500rpm Stirring was continued at a speed of 10 minutes, and then the mixture was rapidly cooled to 20 ° C, washed with anhydrous ether 4 times, and then pouring method at 300-G bar under the action of an external magnetic field, the non-emulsified magnetite precipitation and Doxorubicin magnetic albumin microspheres separation, and finally the magnetic albumin microsphere made