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目的建立完善癫痫平片的质量控制标准。方法用薄层色谱法鉴别癫痫平片中柴胡、猪牙皂,用HPLC法测定方中君药石菖蒲有效成分β-细辛醚的含量。结果薄层鉴别中,柴胡和猪牙皂供试品分别在与其对照药材相对保留时间的相同位置上,显相同颜色的斑点,且阴性无干扰;HPLC法测得β-细辛醚进样量0.212～2.65μg与峰面积的线性关系良好,回归方程为:Y=2.045×103X+10.430(r=0.9999),平均回收率为96.7%,RSD=1.08%。结论所用方法简便可行、重复性好,可用于癫痫平片的质量控制。
Objective To establish and improve the quality control standard of epilepsy plain film. Methods Thin layer chromatography was used to identify Bupleurum chinense and pig tooth soap in Epilepsy Tablets. The content of β-asarone in Corydalis intermedius was determined by HPLC. Results In the TLC identification, the Bupleurum and pig tooth soap samples showed the same color spots at the same relative retention time as the reference materials, respectively, and had no negative interference. The β-asarone injection volume The linear relationship between 0.212 ~ 2.65μg and peak area was good. The regression equation was Y = 2.045 × 103X + 10.430 (r = 0.9999). The average recovery was 96.7% and RSD was 1.08%. Conclusion The method is simple and feasible, reproducible, and can be used for the quality control of epilepsy plain film.