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目的 体外大量扩增和纯化具有典型表型、形态和功能的树突状细胞（ＤＣ），以进行相关基础研究和临床应用。方法 采用免疫磁珠法分离脐血ＣＤ３４ ＋ 细胞及外周血去Ｂ、去Ｔ淋巴细胞的单个核细胞（ 单核细胞） ，然后以ＧＭＣＳＦ、ＩＬ４、ＴＮＦα、Ｆｌｔ３ 配基（ＦＬ）、ＳＣＦ等不同的细胞因子配伍，分别诱生ＤＣ，通过流式细胞仪、电镜、光镜分析其特性，同时检测其刺激同种Ｔ细胞增殖的能力。结果 脐血与外周血诱生ＤＣ的方案不同，由脐血ＣＤ３４＋ 细胞诱导ＤＣ 时，ＧＭＣＳＦ＋ ＴＮＦα＋ ＳＣＦ＋ ＦＬ 组合可使ＣＤ１ａ＋ 细胞比例增至（２７ ．１８ ±１－５６）％ ，明显高于单独应用ＧＭＣＳＦ组［（０．６５±０ ．３８）％ ］ 。外周血单核细胞诱导的ＤＣ，则ＧＭＣＳＦ＋ 高剂量ＩＬ４（１０００ Ｕ／ｍｌ） 组合效率最高，诱生的ＣＤ１ａ ＋ 细胞可达（２１ ．８０ ±０－３２） ％ 。两种来源的ＤＣ在表型及形态上差异无显著性，两者同样具有刺激同种异体淋巴细胞增殖的能力。结论 从脐血和外周血均可诱生ＤＣ，根据应用目的的不同对其进行选择，这为ＤＣ用于临床治疗选择不同细胞来源提供了实验基础。
Objective To extensively amplify and purify dendritic cells (DCs) with typical phenotypes, morphologies and functions in vitro for basic research and clinical applications. Methods Immunomagnetic beads method was used to isolate cord blood CD34 + cells and peripheral blood to go to B, mononuclear cells (monocytes) to T lymphocytes, and then to GM CSF, IL 4, TNF α, Flt3 ligand ( FL), SCF and other different cytokines, induce DCs, analyze their characteristics by flow cytometry, electron microscope and light microscope, and test their ability to stimulate the proliferation of allogeneic T cells. Results Cord blood and peripheral blood of DC induced by different programs, induced by cord blood CD34 + DC, GM CSF TNF α + SCF + FL combination of CD1a + cell ratio increased to (27.18 ± 1-56)%, significantly Higher than GM CSF alone group [(0.65 ± 0.38)%]. Monocytes induced by peripheral blood mononuclear cells, GM CSF + high dose of IL 4 (1000 U / ml) combination of the highest efficiency, induced CD1a + cells (21 .80 ± 0-32)%. The two sources of DC in the phenotype and morphological differences were not significant, both also have the ability to stimulate allogenic lymphocyte proliferation. Conclusion Both DC and DC can be induced from both cord blood and peripheral blood, which can be selected according to the purpose of application. This provides an experimental basis for selecting different cell sources for clinical treatment of DC.