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AIM:To study the diagnosis of Helicobacter pylori(H pylori)infection through the determination of serum levels of anti-H pylori IgG and IgA antibodies,and the levels of anti-H pyloriIgA antibodies in duodenal fluid.METHODS:Data were collected from 93 patients submittedto upper digestive endoscopy due to dyspeptic symptoms.The patients were either negative(group A)or positive(group B)to H pylori by means of both histological detectionand urease tests.Before endoscopy,peripheral blood wascollected for the investigation of anti-H pylori IgG andIgA antibodies.To perform the urease test,biopsies wereobtained from the gastric antrum.For the histologicalevaluation,biopsies were collected from the gastric antrum(greater and lesser curvatures)and the gastric body.Following this,duodenal fluid was collected from the firstand second portions of the duodenum.For the serologicalassaying of anti-Hpylori IgG and IgA,and anti-Hpylori IgAin duodenal fluids,the ELISA method was utilized.RESULTS:The concentration of serum IgG showed sensitivityof 64.0%,specificity of 83.7%,positive predictive value of82.0%,negative predictive value of 66.6% and accuracyof 73.1% for the diagnosis of H pylori infection.For thesame purpose,serum IgA showed sensitivity of 72.0%,specificity of 65.9%,positive predictive value of 72.0%,negative predictive value of 67.4% and accuracy of 69.8%.If the serological tests were considered together,i.e.whenboth were positive or negative,the accuracy was 80.0%,sensitivity was 86.6%,specificity was 74.2%,positivepredictive value was 74.2% and negative predictive valuewas 86.6%.When values obtained in the test for detectingIgA in the duodenal fluid were analyzed,no significantdifference(P=0.43)was observed between the valuesobtained from patients with or without H pylori infection.CONCLUSION:The results of serum IgG and IgA tests forH pylori detection when used simultaneously,are moreefficient in accuracy,sensitivity and negative predictive value,than those when used alone.The concentration of IgAantibodies in duodenal fluid is not useful in identifying patientswith or without H pylori.
AIM: To study the diagnosis of Helicobacter pylori (H pylori) infection through the determination of serum levels of anti-H pylori IgG and IgA antibodies, and the levels of anti-H pylori IgA antibodies in duodenal fluid. METHODS: Data were collected from 93 the patients submittedto upper digestive endoscopy due to dyspeptic symptoms. the patients were either negative (group A) or positive (group B) to H pylori by means of both histological detection and urease tests. Endoscopy, peripheral blood wascollected for the investigation of anti-H pylori IgG and IgA antibodies. perform the urease test, biopsies wereobtained from the gastric antrum. For the histologic evaluation, biopsies were collected from the gastric antrum (greater and lesser curvatures) and the gastric body. Published this, duodenal fluid was collected from the firstand second portions of the duodenum. For the serologicalassaying of anti-Hpylori IgG and IgA, and anti-Hpylori IgAin duodenal fluids, the ELISA method was utilized. RESULTS: The conc entration of serum IgG showed sensitivity of 64.0%, specificity of 83.7%, positive predictive value of 82.0%, negative predictive value of 66.6% and accuracy of 73.1% for the diagnosis of H pylori infection. For the purpose of the serum IgA showed sensitivity of 72.0 %, specificity of 65.9%, positive predictive value of 72.0%, negative predictive value of 67.4% and accuracy of 69.8% .If the serological tests were considered together, iewhenboth were positive or negative, the accuracy was 80.0%, the sensitivity was 86.6 %, specificity was 74.2%, positive predictive value was 74.2% and negative predictive value was 86.6% .When values obtained in the test for detectingIgA in the duodenal fluid were analyzed, no significantdifference (P = 0.43) was observed between the valuesobtained from patients with or without H pylori infection. CONCLUSION: The results of serum IgG and IgA tests for H pylori detection when used simultaneously, are more efficient in accuracy, sensitivity and negative predictive value, than those when usedalone. The concentration of IgA antibodies in duodenal fluid is not useful in identifying patients with or without H pylori.