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目的：应用深低温保存的角膜缘干细胞同种异体移植治疗眼表疾病。方法：新鲜供体取穿透性移植片后剩下的角膜缘干细胞行程序降温，深低温保存，４例５眼临床眼表疾病应用深低温保存的角膜缘干细胞移植片行同种异体移植。结果；５眼角膜上皮修复且稳定，上皮平均愈合时间为７．６±５．７天。无感染、排斥等不良反应，除１眼视力无改变外。其余４眼视力均有不同程度提高。结论：深低温保存法能够将穿透性角膜移植剩下的角膜缘干细胞重新利用，为临床角膜缘干细胞移植治疗眼表疾病提供了可利用的材料。
OBJECTIVE: To treat ocular surface diseases with limbal stem cell allograft preserved by cryopreservation. METHODS: The limbal stem cells left by the fresh donor after cryopreservation were cryopreserved and cryopreserved. Four cases of 5 eyes with ocular surface disease underwent cryopreservation with limbal stem cell transplantation for allogeneic transplantation. Results: The corneal epithelium was repaired and stable in 5 eyes. The mean epithelial healing time was 7.6 ± 5.7 days. No infection, rejection and other adverse reactions, except for 1 eyesight without change. The remaining 4 eyes have different degrees of improvement of visual acuity. Conclusion: Cryopreservation method can recycle the limbal stem cells left by penetrating keratoplasty, which provides a useful material for the treatment of ocular surface diseases by limbal stem cell transplantation.