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糖皮质激素经受体介导对间质细胞中睾酮合成行使直接的抑制作用。间质细胞中１１β－羟类固醇脱氢酶（１１β－ＨＳＤ）通过控制细胞内糖皮质激素浓度调节睾酮合成。至今，已鉴定了两种１１β－ＨＳＤ同工酶，Ⅰ型从肝脏中纯化，是以ＮＡＤＰ（Ｈ）为辅酶的氧化还原酶。从肾脏纯化的Ⅱ型，是依赖ＮＡＤ的氧化酶。有关间质细胞中１１β－ＨＳＤ的研究，尤其对其特性的研究尚不深入。本研究通过测定间质细胞中Ⅰ型１１β－ＨＳＤ抗原及其ｍＲＮＡ表达，观察间质细胞中该酶的优势辅酶，纯化的活间质细胞中１１β－ＨＳＤ催化反应类型及间质细胞１１β－ＨＳＤ对糖皮质激素调节的反应等几个方面来评价间质细胞中１１β－ＨＳＤ的特性。结果发现，间质细胞显示Ⅰ型１１β－ＨＳＤ抗原及其ｍＲＮＡ的表达（ＲＴ－ＰＣＲ产物，６９６ｂｐ），未见有Ⅱ型ｍＲＮＡ表达。间质细胞中该酶是以ＮＡＤＰ（Ｈ）为优势辅酶（Ｐ＜０．０５），以氧化反应为优势的氧化还原酶（Ｐ＜０．０５）。切除肾上腺后大鼠其纯化的活间质细胞及培养的间质细胞１１β－ＨＳＤ氧化酶活性受糖皮质激素调节（Ｐ＜０．０５）。本研究表明，大鼠间质细胞中１１β－ＨＳＤ是一个以催化氧化反应为优势，以ＮＡＤＰ（Ｈ）为辅酶的Ⅰ型同工酶，?
Glucocorticoid receptor-mediated direct inhibition of testosterone synthesis in stromal cells. 11β-hydroxysteroid dehydrogenase (11β-HSD) in stromal cells regulates testosterone synthesis by controlling intracellular glucocorticoid concentrations. To date, two 11 [beta] -HSD isozymes have been identified, type I purified from the liver, an oxidoreductase that uses NADP (H) as a coenzyme. Type II, purified from the kidney, is an NAD-dependent oxidase. The study of 11β-HSD in stromal cells, especially its characteristics, is not yet in-depth. In this study, the expression of type 1 11β-HSD antigen and its mRNA in stromal cells was measured to observe the type of 11β-HSD catalytic reaction and the expression of 11β-HSD in mesenchymal cells Response to glucocorticoid regulation and several other aspects to evaluate the characteristics of 11β-HSD in stromal cells. The results showed that type I 11β-HSD antigen and its mRNA expression (RT-PCR product, 696bp) showed no expression of type II mRNA in stromal cells. The stromal cells in the enzyme is NADP (H) for the coenzyme (P <0.05), the oxidation reaction is the dominant oxidoreductase (P <0.05). The 11β-HSD oxidase activity of purified live mesenchymal cells and cultured stromal cells in rats after adrenalectomy was regulated by glucocorticoid (P <0.05). The present study shows that 11β-HSD in rat stromal cells is a type I isoenzyme that catalyzes the oxidation reaction predominantly with NADP (H) as a coenzyme.