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目的探讨人canstatin基因对人食管癌裸鼠移植瘤的的影响作用,寻求其抑制肿瘤生长的机制。方法 KYSE150细胞建立裸鼠食管癌动物模型,Ad-canstatin实施干预,RT-PCR检测肿瘤组织bcl-xl、cy-clinD1的mRNA的表达水平。免疫组织化学方法检测微血管密度(MVD)。结果治疗组肿瘤体积明显小于两个对照组,差异有统计学意义(P<0.05),而两对照组间差异无统计学意义。对电泳结果进行灰度分析和统计学分析显示canstatin治疗组cyclinD1、bcl-xl的mRNA表达明显低于GFP组和PBS组,差异均有统计学意义(P<0.05)。canstatin治疗组MVD明显低于对照组。结论人canstatin基因对人食管癌移植瘤的生长具有抑制作用,其作用机制可能与抑制cyclin D1、bcl-xl表达而抑制肿瘤血管生成有关。
Objective To investigate the effect of human canstatin gene on human esophageal carcinoma xenografts in nude mice and to find out its mechanism of inhibiting tumor growth. Methods KYSE150 cells were used to establish animal model of esophageal cancer in nude mice. Interfered with Ad-canstatin, the mRNA expression of bcl-xl, cy-clinD1 in tumor tissues was detected by RT-PCR. Immunohistochemistry was used to detect microvessel density (MVD). Results The tumor volume in the treatment group was significantly smaller than that in the two control groups (P <0.05), but there was no significant difference between the two control groups. The gray scale analysis and statistical analysis of electrophoresis results showed that the mRNA expression of cyclinD1 and bcl-xl in canstatin treatment group was significantly lower than that in GFP group and PBS group (P <0.05). The MVD in canstatin group was significantly lower than that in control group. Conclusion The human canstatin gene has an inhibitory effect on the growth of human esophageal carcinoma and its mechanism may be related to the inhibition of the expression of cyclin D1 and bcl-xl and the inhibition of tumor angiogenesis.