论文部分内容阅读
采用微生物分离培养技术和巢式PCR技术相结合方法对2011年采自广西北海南万和古城、钦州茅岭和钦州港、防城港企沙和公车的拟穴青蟹(Scylla paramamosain)携带寄生虫、细菌和病毒状况开展调查。寄生虫检测结果显示,仅钦州茅岭拟穴青蟹鳃组织中发现寄生虫茗荷(儿)。细菌性病原体检测结果显示,共有11种病原性细菌存在于健康养殖拟穴青蟹体内,弧菌属细菌性病原出现频率最高,其中副溶血弧菌和溶藻弧菌在调查区域内均有分布,其他菌种则呈现一定的地域特异性。病毒性病原体检测结果显示,白斑综合症病毒仅在钦州茅岭拟穴青蟹检出,阳性率为4.94%;双顺反子病毒于北海古城和钦州茅岭拟穴青蟹检出阳性携带率为3.70%;呼肠孤病毒携带率则较高,北海、防城港以及钦州拟穴青蟹均有检出,阳性率达25.93%。由此可见,10-11月广西区内健康养殖拟穴青蟹携带弧菌和呼肠孤病毒的比例远远高于其他病原体,为拟穴青蟹携带的主要病原体。
Microbial isolation techniques and nested PCR were used to carry parasites in Scylla paramamosain collected from Nanwanhe Ancient City, Qinzhou Maoling and Qinzhou Ports, Fangchenggang Qisha and Buses in 2011 in Beihai, Bacteria and virus status investigation. The results of parasite detection showed that only the parasites Minglin (children) were found in the gill tissues of the green crab in Qinzhou Maolin. Bacterial pathogens test results showed that a total of 11 kinds of pathogenic bacteria exist in the healthy breeding of acanthopanax crab body, the highest frequency of bacterial pathogens Vibrio, which Vibrio parahaemolyticus and Vibrio alginolyticus in the survey area are distributed , Other species showed a certain geographical specificity. Viral pathogen test results showed that white spot syndrome virus was detected only in Qinzhou Maoling quasi-point crab, the positive rate was 4.94%; bicistronic virus in the ancient city of Beihai and Qinzhou Maoling quasi-point crab detection positive carrying rate Was 3.70%. The reovirus carrying rate was higher in Beihai, Fangchenggang and Qinzhou quarantine sites, with a positive rate of 25.93%. Thus, from October to November in Guangxi Province, the ratio of the number of live-borne crabs carrying vibrio and reovirus in farmed farmed crabs is much higher than that of other pathogens.