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应用生物素标记HBV DNA(乙肝病毒脱氧核糖核酸)作探针,对129例肝病患者肝组织作原位杂交研究。发现HBV DNA主要存在肝细胞浆内,可分为胞浆致密型、疏松型和包涵体型。HBV DNA阳性肝细胞在肝实质中分为三种型:小叶型、局灶型与散在点状分布。HBV DNA在慢性活动型肝炎中检出率最高(81％),显著高于肝硬化,慢性小叶型肝炎、急性肝炎及原发性肝癌组。乙肝复制指标阳性患者肝细胞内HBV DNA检出率明显高于非复制组;并观察到HBV DNA阳性肝细胞与肝细胞坏死灶关系密切,多数紧紧毗邻肝细胞坏死灶/或和位于坏死灶中间,尤以局灶型分布的HBV DNA阳性肝细胞为显著。
Biotinylated HBV DNA (HBV DNA) was used as a probe to study the in situ hybridization of 129 liver tissues from patients with liver disease. HBV DNA was found mainly in the liver cytoplasm, can be divided into the cytoplasm of dense, loose and inclusion body. HBV DNA-positive hepatocytes are divided into three types in the liver parenchyma: lobular, focal and scattered dot-like distribution. The detection rate of HBV DNA was the highest in chronic active hepatitis (81%), which was significantly higher than that of cirrhosis, chronic small-cell hepatitis, acute hepatitis and primary liver cancer. The detection rate of HBV DNA in hepatocytes was significantly higher in HBsAg-positive patients than that in non-duplicated ones. HBV DNA-positive hepatocytes were found to be closely associated with hepatocellular necrosis, most closely adjacent to hepatocellular necrosis / or in necrotic lesions In the middle, especially in the distribution of HBV DNA positive hepatocytes was significant.