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利用抗ＣＤ３４单克隆抗体吸附磁性微球的方法分离纯化脐带血ＣＤ３４＋细胞，将其种入照射后的成年骨髓基质。比较ｒｈＧＭ－ＣＳＦ、ＩＬ－３及两者的联合对植入效率的促进作用。结果表明：经２ｈ铺展贴壁后，对照组只有３６％的ＣＤ３４＋细胞植入基质，而生长因子预处理组则有６８—８９．６％的ＣＤ３４＋细胞植入基质。在长期液体培养体系中则显示了植入ＣＤ３４＋细胞多的处理组造血重建快速而持久。表明ＧＭ－ＣＳＦ和ＩＬ－３预处理将明显提高脐带血移植效率。
Umbilical cord blood CD34 + cells were isolated and purified by using anti-CD34 monoclonal antibody to adsorb magnetic microspheres, which were then seeded into the irradiated adult bone marrow stroma. The effects of rhGM-CSF, IL-3 and their combinations on the implantation efficiency were compared. The results showed that only 36% of the CD34 + cells in the control group were implanted into the stroma after 2 h of spreading, while 68-89.6% of the CD34 + cells in the growth factor pretreatment group were implanted into the stroma. In the long-term liquid culture system, hematopoietic reconstitution was shown to be fast and long-lasting in the treatment group with more CD34 + cells. It is indicated that pretreatment with GM-CSF and IL-3 will significantly improve the efficiency of cord blood transplantation.