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目的建立检查中药洗剂中非法添加肾上腺皮质激素的LC-MS方法。方法采用液相色谱-三重四极杆质谱联用仪同时检测中药洗剂中非法添加16种肾上腺皮质激素类化学成分。进行定性和定量分析,并进行方法学验证。结果 16种常被用于非法添加的肾上腺皮质激素类药物在色谱柱中得到了良好的分离,再配合产物离子质谱图进行定性,利用多反应监测模式的峰面积进行定量计算,专属性强,精密度良好,回收率和线性关系满足分析要求。结论该方法简便、准确,可以用于中药洗剂中非法添加肾上腺皮质激素类化学成分的实验室筛查。
Objective To establish an LC-MS method to check the illegal addition of adrenocorticotropic hormone in traditional Chinese medicine lotion. Methods Sixteen adrenocorticotropic hormone chemicals were illegally added to Chinese lotion by liquid chromatography-triple quadrupole mass spectrometry. Qualitative and quantitative analysis, and methodological verification. Results 16 kinds of corticoids which were often added illegally were well separated in the chromatographic column and qualitatively combined with the ion chromatogram of the product. Quantitative calculation was made by using the peak area of the multi-reaction monitoring mode. Good precision, recovery and linear relationship to meet the analytical requirements. Conclusion The method is simple and accurate and can be used in laboratory screening of Chinese herbal medicine lotion with illegally added adrenocorticoid chemical components.