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目的:制定一种简便、迅速、准确的银杏内酯含量测定方法,准确测定不同银杏叶提取物中4种银杏内酯的含量。方法:经处理过的银杏叶提取物采用HPLC-ELSD法测定其银杏内酯的含量。流动相为甲醇-水(25∶75);流速:1.0ml.min-1;ELSD检测器;漂移管温度:104℃;载气流速:2.9L·min-1;柱温:25℃。结果:建立的方法简便、准确、灵敏度高,能很好地控制银杏叶提取物的质量。所测不同厂家生产的银杏叶提取物中银杏内酯的含量均符合《中国药典》(2005版一部)的要求。
Objective: To develop a simple, rapid and accurate method for determination of ginkgolides and accurately determine the contents of 4 ginkgolides in different ginkgo leaf extracts. Methods: The content of ginkgo lactone was determined by HPLC-ELSD method of treated Ginkgo biloba extract. The mobile phase was methanol-water (25:75); flow rate: 1.0 ml.min-1; ELSD detector; drift tube temperature: 104°C; carrier gas flow rate: 2.9L·min-1; column temperature: 25°C. Results: The established method was simple, accurate, and highly sensitive, and it could well control the quality of Ginkgo biloba extract. The contents of ginkgolide in Ginkgo biloba extracts produced by different manufacturers were all in compliance with the requirements of the “Chinese Pharmacopoeia” (2005 edition).