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目的观察人白细胞干扰素（ＩＦＮα）对我们建立的子宫内膜腺癌细胞系（ＪＥＣ）的细胞生长和细胞周期的影响。方法将不同浓度：２０００ｕ／ｍｌ、５００ｕ／ｍｌ、１００ｕ／ｍｌ的ＩＦＮα与ＪＥＣ共同培养７天，检测ＪＥＣ细胞生长曲线、细胞的凝集素受体表达，并用流式细胞仪分析细胞周期的变化。结果和结论ＩＦＮα对ＪＥＣ细胞的增殖有抑制作用，浓度越大，抑制作用越明显，但不影响凝集素受体的表达；ＩＦＮα可使ＪＥＣ细胞Ｇ１／Ｇ０期增加、Ｓ期增加和Ｇ２／Ｍ期阻滞，前者与ＩＦＮα浓度呈正相关，而后者则为负相关。
Objective To observe the effects of human interferon (IFNα) on the cell growth and cell cycle of endometrial adenocarcinoma cell line (JEC). Methods Different concentrations of 2000u / ml, 500u / ml, 100u / ml of IFN α and JEC co-cultured for 7 days to detect JEC cell growth curve, the expression of lectin receptors, and flow cytometry analysis of cell cycle Variety. RESULTS AND CONCLUSION: IFNα inhibited the proliferation of JEC cells. The greater the concentration, the more obvious the inhibitory effect was, but it did not affect the expression of lectin receptor. IFNα increased the G1 / G0 phase and increased the S phase in JEC cells And G2 / M phase arrest, the former was positively correlated with IFN-α concentration, while the latter was negatively correlated.