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用改进的酚-氯仿抽提法分离615小鼠肝脏总核酸,再用Oligo(dT)-纤维素亲和层析法,从总核酸中分离得到纯度较高的带Poly(A)的mRNA组分。经体外无细胞蛋白合成系统的检测及产物的免疫沉淀反应,都表明所分离的mRNA具有生物活性。本法操作简便,并有不受高速离心条件限制的特点。
The purified total RNA of liver of 615 mice was isolated by modified phenol-chloroform extraction method, and the poly (A) mRNA group with high purity was isolated from the total nucleic acid by Oligo (dT) -cellulose affinity chromatography Minute. Detection of the in vitro cell-free protein synthesis system and immunoprecipitation of the product all indicate that the isolated mRNA is biologically active. This method is easy to operate and has characteristics that are not limited by high-speed centrifugation.