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目的:探索大鼠局灶性脑缺血再灌注损伤最严重的时间点,确定开展发病机制及药理学研究最合适的时间窗。方法:制造线栓法阻塞大脑中动脉致大鼠局灶性脑缺血再灌注损伤模型(MCAO),缺血后2h再灌注,术后动态观察大鼠的状态,神经行为学,脑梗死比率和血液中的相关生化指标。结果:模型大鼠的病理损害具有明显的时相性,随着时间的推移逐渐加重,于48h达到高峰,96h时病理损害仍然存在,但明显减弱;在脑缺血第48h时,脑梗死比率、神经行为学和血清中脂质过氧化指标等病理损害均比较严重,且动物的体重和状态开始下降。结论:MCAO大鼠脑缺血后第48h时最适宜于开展发病机制及药理学研究,且雄性SD大鼠更适宜制作该模型。
OBJECTIVE: To explore the most time-point of focal cerebral ischemia-reperfusion injury in rats and to determine the most suitable time window for studying pathogenesis and pharmacology. Methods: MCAO was induced by occlusion of middle cerebral artery (MCAO) in rats by occlusion of the middle cerebral artery occlusion (MCAO), and then reperfused at 2 hours after ischemia. The dynamic changes of neurological behavior, cerebral infarction rate And blood related biochemical indicators. Results: The histopathological damage of the model rats was obviously time-phasic and gradually increased with the passage of time. The peak value reached the peak at 48 hours and the pathological damage still existed at 96 hours, but obviously decreased. At the 48th hour after cerebral ischemia, the infarction rate, Neurobehavioral and serum lipid peroxidation and other pathological lesions are more serious, and the animals began to decline in body weight and status. Conclusion: MCAO is the most suitable for studying the pathogenesis and pharmacology at 48h after cerebral ischemia. And it is more suitable for male SD rats to make this model.