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目的观察不同剂量的茶多酚 (TP)、维生素C(VitC)对FeSO4 半胱氨酸诱发离体人血浆脂质过氧化及CCl4诱发肝自由基损伤的抑制作用。方法 ( 1 )在FeSO4 半胱氨酸诱发离体人血浆脂质过氧化损伤体系中 ,分别检测 0 .3、0 .9、2 .7、8.1mg/L(C～F组 )的TP及 3、9、2 7、81mg/L(G～J组 )的VitC对该体系过氧化脂质 (MDA)生成的影响 ;( 2 )雄性昆明种小鼠 36只 ,随机分为 4组 (n =9) ,分别为空白对照组 (A)、单纯损伤组 (B)、茶多酚 1 0 0mg/kg组 (C) ,以及VitC 1 0 0mg/kg组 (D)。除空白对照外 ,用药组以相应剂量的防护药物灌胃 1次 /日 ,共 3次。末次灌胃 1 2h后 ,除空白对照外 ,其他 3组均用 2 30mg/kg的CCl4灌胃 ,36h后 ,各组小鼠均断头处死 ,分别检测肝匀浆中MDA的含量。结果TP、VitC均可剂量相关地抑制FeSO4 半胱氨酸诱发的离体人血浆脂质过氧化损伤 ,其中TP抑制率分别为30 .7% ,32 .0 %、46.9%、59.7% ;VitC的抑制率分别为 8.3%、41 .4%、47.7%、52 .7%。在CCl4诱发肝自由基损伤体系中 ,同等剂量的TP、VitC对MDA的抑制率分别为 45.2 %、42 .8%。结论在FeSO4 半胱氨酸诱发的人离体血浆过氧化损伤体系中 ,TP( 0 .3～ 8.1mg/L)及VitC( 9～ 81mg/L)对脂质过氧化均有明显的抑制作用 (P <0 .0 1 ) ;同等剂量下 ,T
Objective To observe the inhibitory effects of different doses of tea polyphenols (TP) and vitamin C on lipid peroxidation induced by FeSO4 cysteine in human plasma and liver injury induced by CCl4. Methods (1) In the system of FeSO4 cysteine-induced human plasma lipid peroxidation injury, the TP of 0.3, 0.09, 2.7, 8.1 mg / L (C ~ F) (2) 36 male Kunming mice were randomly divided into 4 groups (n = 3, 9, 27, 81mg / L VitC group) = 9) were blank control group (A), simple injury group (B), tea polyphenol 100 mg / kg group (C) and VitC 100 mg / kg group (D). In addition to the blank control, the medication group to the appropriate dose of protective drug gavage 1 times / day, a total of 3 times. After the last intragastric administration of 12 h, except the blank control, the other three groups were fed with 30 mg / kg of CCl4. After 36 h, the mice in each group were decapitated, and the content of MDA in the liver homogenate was detected respectively. Results Both TP and VitC could inhibit the lipid peroxidation induced by FeSO4 cysteine in vitro. The TP inhibition rates were 30.7%, 32.0%, 46.9% and 59.7%, respectively. VitC The inhibition rates were 8.3%, 41.4%, 47.7% and 52.7% respectively. In CCl4-induced liver injury system, the same dose of TP, VitC inhibition of MDA were 45.2%, 42.8%. Conclusions TP (0.3-3.1 mg / L) and VitC (9-81 mg / L) significantly inhibit lipid peroxidation in human plasma induced by FeSO4 cysteine, (P <0 01); the same dose, T