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为检测水稻纹枯病抗性基因位点,采用牙签接种法对籼稻9311和粳稻日本晴以及由它们构建的119个染色体单片段置换系进行纹枯病抗性鉴定,并使用该群体的分子连锁图谱进行数量性状座位(QTL)分析。共检测到3个纹枯病相关QTL(qsb8-1,qsb8-2和qsb8-3),分别位于第8染色体相邻标记RM3262、RM5485和RM3496附近,所在遗传区间分别为81.7cM~91.7cM、91.7cM~108.1cM和108.1cM~119.6cM。其中qsb8-2的加性效应为负值,表明感病亲本携带的该片段可以增强纹枯病抗性;qsb8-1和qsb8-3的加性效应为正值,表明感病亲本携带的该片段减弱了纹枯病抗性。
In order to detect rice sheath blight resistance gene locus, the resistance to sheath blight of indica rice 9311 and japonica rice Nipponbare and the 119 chromosomal single-site substitution lines constructed by them were identified by toothpick inoculation, and the molecular linkage map Quantitative trait locus (QTL) analysis. Three QTLs for sheath blight (qsb8-1, qsb8-2 and qsb8-3) were detected in the vicinity of RM3262, RM5485 and RM3496 on chromosome 8, with the genetic intervals of 81.7cM to 91.7cM, 91.7 cM-108.1 cM and 108.1 cM-119.6 cM. Among them, the additive effect of qsb8-2 was negative, indicating that this fragment carried by the susceptible parent could enhance the resistance to sheath blight. The additive effect of qsb8-1 and qsb8-3 was positive, indicating that the susceptible parent carrying this Fragmentation attenuated sheath blight resistance.