Effects of sodium arsenite on Cyclin D1 and Cyclin dependent kinase 4 of human normal liver cells

• 作 者: 胡婷
• 机 构: Dept Toxicol,Guizhou Med Univ,
• 出 处: China Medical Abstracts(Internal Medicine)
• 发表日期: 2017年02期
• 关键词: Cyclin cytometry exposed doses cycle statis creased ultimately proportion

Objective To investigate the effects of sodium arsenite(NaAsO_2)on the expressions of Cyclin D1 and Cyclin dependent kinase 4(CDK4)in human normal liver cells(L-02).Methods L-02 cells were exposed to differ-ent doses of NaAsO_2(0,50,100,150μmol/L)for 24 h,flow cytometry was used to detect the cell cycle,real time quantitative PCR and Western blotting were used to detect the Cyclin D1,CDK4 mRNA and protein expres-

Objective To investigate the effects of sodium arsenite (NaAsO_2) on the expressions of Cyclin D1 and Cyclin dependent kinase 4 (CDK4) in human normal liver cells (L-02) .Methods L-02 cells were exposed to differ- ent doses of NaAsO_2 (0, 50, 100, 150 μmol / L) for 24 h, flow cytometry was used to detect the cell cycle, real time quantitative PCR and Western blotting were used to detect the cyclin D1, CDK4 mRNA and protein expres-