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目的分析1例天津地区夏季季节性H3N2亚型流感感染病例中NA基因的变异情况。方法分离病毒毒株,提取核酸,经逆转录聚合酶链反应(RT-PCR)方法扩增NA基因,获得的测序结果与中国地区各地毒株进行同源比对,使用生物信息软件绘制种系发生树,翻译后序列与WHO推荐的2014年-2015年疫苗株A/Texas/50/2012的NA氨基酸序列进行比对。结果 NA基因进化树分析表明,该毒株与2011年-2013年广东、杭州、中山等地区毒株集中于一支,与2014年-2015年疫苗株比对共有3个位点的氨基酸发生变化。该毒株未出现耐药位点的变异。结论本次研究所分离毒株与2014年-2015年疫苗株比对,其NA基因有变异,但未发现耐药位点的变异。
Objective To analyze the variation of NA gene in a case of seasonal seasonal H3N2 influenza infection in Tianjin area. Methods The virus strains were isolated and the nucleic acids were extracted. The NA gene was amplified by reverse transcription-polymerase chain reaction (RT-PCR). The obtained sequencing results were homologous compared with those in different parts of China. The resulting tree and posttranslational sequences were aligned with NA amino acid sequences of the WHO-recommended 2014-2015 vaccine strain A / Texas / 50/2012. Results The phylogenetic tree analysis of NA gene showed that the strain was concentrated in Guangdong, Hangzhou and Zhongshan from 2011 to 2013. There were three amino acid changes in the strains from 2014 to 2015 . The strain did not show the variation of drug-resistant loci. Conclusion The strains isolated in this study were more variable than the vaccine strains from 2014 to 2015. However, no variation of the drug-resistant loci was found.