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目的 制备氰基丙烯酸正丁酯胰岛素纳米粒 (insulinnanoparticles INP) ,研究其理化特性 ,观察 sc和 po给药对糖尿病大鼠的降血糖作用 .方法 用改良的乳液聚合法制备氰基丙烯酸正丁酯胰岛素纳米粒 ,用透射电子显微镜观察 INP的大小、形态 ,用高压液相色谱法 (HPL C)测定其包裹率 ,用 ip四氧嘧啶制备糖尿病大鼠模型 .血糖用快速血糖仪测定 .用 t检验和 χ2 检验进行统计学处理 .结果 用改良的乳液聚合法制得的 INP粒径为 (30± 0 .5 ) nm,包裹率为 95 % ,给糖尿病大鼠 sc30 U·kg- 1 ,INP组 1h开始起作用 ,4～ 6 h达峰值 ,血糖降低 98% ,作用持续 2 4h,而普通胰岛素组 1h达峰值 ,最大降血糖幅度为 5 5 % ,作用持续 4h. sc 2 0 U· kg- 1 ,INP组1h开始起作用 ,4～ 6 h达峰值 ,最大降血糖幅度为 74% ,作用持续 2 0 h,而鱼精蛋白锌胰岛素组 1h开始起作用 ,4～ 6 h达峰值 ,最大降血糖幅度为 41% ,作用也持续 2 0 h.给糖尿病大鼠 po INP 12 0 U· kg- 1 ,1d后空腹血糖开始下降 ,3d效果最佳 ,血糖降低 89% ,5～ 7d后恢复高血糖 ,而 po普通胰岛素组血糖无明显变化 ,2～ 4d的血糖两组比较相差显著 (P<0 .0 5 ) . 5 0 U· kg- 1 组及 10 0 U· kg- 1 组作用持续时间均为 5d,12 0 U· kg- 1 组的作用时间为 7d.三
OBJECTIVE To prepare insulin nanoparticles INP, study its physical and chemical properties, and observe the hypoglycemic effect of sc and po on diabetic rats.Methods A modified emulsion polymerization was used to prepare n-butyl cyanoacrylate Insulin nanoparticles were observed by transmission electron microscopy INP size, morphology, using high pressure liquid chromatography (HPL C) determination of the package rate, with ip alloxan prepared diabetic rat model.Determination of fasting blood glucose using t Test and χ2 test.Results The particle size of INP prepared by modified emulsion polymerization was (30 ± 0.5) nm and the encapsulation efficiency was 95% 1 h began to work, 4 to 6 h peak, blood sugar decreased 98%, the role of sustained 24 h, while the normal insulin group peaked at 1 h, the maximum hypoglycemic amplitude was 5 5%, the role of sustained 4 h sc 2 0 U · kg- 1, the INP group started to function at 1h, peaked at 4 ~ 6h, the maximum hypoglycemic amplitude was 74%, the effect lasted 20 h, while the protamine zinc-insulin group started to work at 1h and peaked at 4 ~ 6h Hypoglycemic rate of 41%, the role also Continued for 20 h. The fasting blood glucose began to decline after po INP 12 U · kg ~ (-1) for 1d in diabetic rats, and the best 3d effect was achieved. The blood glucose was decreased by 89% and hyperglycemia was restored after 5 ~ 7d, while the normal insulin group There was no significant difference between the two groups (P <0.05), and the duration of 2 and 4 days had a significant difference between the two groups (P <0.05). The duration of effect of 5 U and 10 U · kg- · Kg-1 group of the role of time for 7d. Three