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目的研究丙烯酰胺对大鼠多组织细胞DNA的损伤作用,观察多不饱和脂肪酸二十碳五烯酸(EPA)和二十二碳六烯酸(DHA)对DNA损伤的拮抗效应。方法将健康成年SD大鼠30只随机分成3个组,雌雄各半。第1组为对照组,第2组为染毒组,第3组为染毒+EPA和DHA组。采用注射方式给大鼠丙烯酰胺染毒,应用单细胞凝胶电泳技术检测各组大鼠的周围血淋巴细胞、肝细胞、肺细胞和脑细胞,以探讨丙烯酰胺所致组织细胞DNA的损伤及EPA和DHA对其的拮抗效应。结果丙烯酰胺染毒组大鼠周围血淋巴细胞、肝细胞、肺细胞和脑细胞尾DNA%均高于对照组,而丙烯酰胺染毒+EPA和DHA组大鼠周围血淋巴细胞、肝细胞、肺细胞及脑细胞尾长、尾DNA%与对照组相比变化不明显。结论丙烯酰胺可致大鼠多组织细胞DNA损伤,EPA和DHA可拮抗这种损伤。
Objective To investigate the effect of acrylamide on DNA damage in rat multi-tissue cells and to observe the antagonistic effect of polyunsaturated fatty acids eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) on DNA damage. Methods Thirty healthy adult SD rats were randomly divided into three groups, male and female. The first group is the control group, the second group is the exposure group, and the third is the exposure + EPA and DHA groups. The rats were injected with acrylamide by injection, and the peripheral blood lymphocytes, hepatocytes, lung cells and brain cells of each group were detected by single cell gel electrophoresis to explore the DNA damage of the tissue cells induced by acrylamide and Antagonism of EPA and DHA to them. RESULTS: The percentages of tail DNA of peripheral blood lymphocytes, hepatocytes, lung cells and brain cells in the acrylamide group were higher than those in the control group. However, in the acrylamide group + EPA and DHA groups, peripheral blood lymphocytes, hepatocytes, Lung cells and brain cells tail length, tail DNA% compared with the control group did not change significantly. Conclusion Acrylamide can cause DNA damage in rat multi-tissue cells, and EPA and DHA can antagonize this damage.