论文部分内容阅读
目的 阐明机体免疫功能的放烧复合效应 ,为放烧复合伤免疫功能障碍的调控提供依据。方法 于不同时相点测定放烧复合伤小鼠 (5Gyγ射线照射合并 12 %Ⅱ度烧伤 )的脾体比 ,脾脏组织学观察 ,分离脾脏T细胞 ,RT PCR检测其IFN γ和IL 4的基因表达水平。结果 ①伤后放烧组的脾脏指数和组织结构的恢复明显快于单放组 ;②放射损伤后T细胞IFN γ和IL 4的基因表达均受到抑制 ,但与IL 4比较 ,IFN γ表达量的降幅小、恢复快 ;③放射损伤后脾脏指数和T细胞IL 4的基因表达呈双向变化 ;④放烧组IFN γ表达量明显高于单放组 ,而两伤组IL 4表达量无区别。结论 ①放射损伤是放烧复合伤的主导损伤因素 ;②IL 4的辐射敏感性高于IFN γ ;③烧伤因素能促进放烧复合伤后脾脏淋巴细胞的增殖及IFN γ的表达 ,不能促进IL 4的表达 ;④受照机体内仍然存在着促进IL 4表达恢复的有效机理
Objective To elucidate the combination effect of the body’s immune function with burn and burn, to provide the basis for the regulation of immune dysfunction caused by burn and burn injury. Methods Spleen and body weight were measured at different time points in spleen and lung of burn-combined injury mice (5 Gy γ-ray irradiation combined with 12% grade Ⅱ burn), splenic T cells were isolated and splenic T cells were isolated. RT-PCR was used to detect IFN γ and IL 4 genes The expression level. Results ① The recovery of spleen index and histological structure of radiotherapy group was significantly faster than that of radiotherapy alone group; ②The mRNA expression of IFN-γ and IL-4 of T cells was inhibited after radiation injury, but compared with IL 4, ; ③ The gene expression of IL-4 in spleen and T cells changed in a bidirectional manner after radiation injury; ④ The expression of IFN-γ in Caofan group was significantly higher than that in single injury group, while there was no difference in IL 4 expression between the two injury groups . Conclusions ① Radiation injury is the main factor of injury in combination with burn injury. ② The radiation sensitivity of IL 4 is higher than that of IFN γ. ③ Burns can promote the proliferation and IFNγ expression of spleen lymphocytes after burn and burn injury, and can not promote IL 4 The expression of IL-4 in the irradiated tissues still exists an effective mechanism