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目的:探讨血管内皮生长因子(VEGF)及其胎肝激酶受体(flk-1)在鼻内翻性乳头状瘤(NIP)恶变前不同阶段中的表达情况。方法:应用免疫组织化学LSAB法检测NIP、非典型增生的不同阶段及恶变组织中VEGF及其受体flk-1的表达情况。应用图像分析仪对染色结果进行定量分析。结果:VEGF、flk-1阳性染色随着不典型增生程度的加重,其在上皮组织和间质结缔组织中的表达强度和范围也增加。轻度不典型增生与NIP比较,中-重度不典型增生与轻度不典型增生比较,VEGF、flk-1表达差异均有统计学意义(均P<0.01)。中-重度不典型增生组织的表达强度与鳞状细胞癌组织接近。结论:VEGF参与了NIP的病理过程,VEGF及其受体flk-1的表达随肿瘤异型程度的增加其表达强度增加,其表达水平可以作为衡量NIP恶变倾向的指标之一。
Objective: To investigate the expression of vascular endothelial growth factor (VEGF) and its fetal liver kinase receptor (flk-1) in different stages of malignant transformation of nasal inverted papilloma (NIP). Methods: Immunohistochemical LSAB method was used to detect the expression of VEGF and its receptor flk-1 in different stages of NIP, atypical hyperplasia and malignant transformation. Application of image analyzer for quantitative analysis of staining results. Results: The expression of VEGF, flk-1 positive staining increased with the degree of dysplasia in epithelial and interstitial connective tissues. Mild dysplasia compared with NIP, moderate-severe dysplasia and mild dysplasia, VEGF, flk-1 expression differences were statistically significant (all P <0.01). The expression intensity of moderate-severe dysplasia was close to that of squamous cell carcinoma. CONCLUSION: VEGF is involved in the pathological process of NIP. The expression of VEGF and its receptor flk-1 is increased with the increase of tumor atypia. The expression of flk-1 may be one of the indicators to evaluate the malignancy of NIP.