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目的:探讨柚皮素对内毒素(lipopolysaccharide,LPS)诱导小鼠的急性肺损伤(acute lung injury,ALI)保护作用。方法:于小鼠腹腔注射LPS(5mg/kg)复制诱导小鼠的急性肺损伤动物模型。将小鼠随机分为空白组、LPS组、地塞米松组(2mg/kg)、柚皮素高剂量组(40mg/kg)、柚皮素低剂量组(20mg/kg),观察各组肺组织病理学改变,测量肺湿/干重比,支气管肺泡灌洗液中性粒细胞百分比、SOD、肺组织中MPO及炎症因子IL-6、TNF-α含量。结果:柚皮素能有效减轻LPS所致肺组织病理学变化,降低肺泡灌洗液中炎症细胞的数目,显著降低肺湿/干重比重,提高SOD水平,并能降低MPO水平。结论:柚皮素可减轻LPS所致急性肺组织损伤,对LPS诱导的急性肺损伤有保护作用。
Objective: To investigate the protective effect of naringenin on acute lung injury (ALI) induced by lipopolysaccharide (LPS) in mice. Methods: The model of acute lung injury was induced in mice by intraperitoneal injection of LPS (5mg / kg). The mice were randomly divided into blank group, LPS group, dexamethasone group (2mg / kg), naringenin high dose group (40mg / kg) and naringenin low dose group (20mg / kg) Histopathological changes, lung wet / dry weight ratio, the percentage of neutrophil in bronchoalveolar lavage fluid, SOD, the levels of MPO and inflammatory cytokines IL-6, TNF-αin lung tissue were measured. Results: Naringenin can effectively reduce the pathological changes of lung tissue induced by LPS, reduce the number of inflammatory cells in BALF, significantly reduce the proportion of lung wet / dry weight, increase the level of SOD and decrease the level of MPO. Conclusion: Naringenin can reduce acute lung injury induced by LPS and protect LPS-induced acute lung injury.