论文部分内容阅读
目的 :通过对口腔鳞癌的手术切除标本划分不同区域 ,研究不同区域的病理分级 ,以及 p53、p2 1基因蛋白表达差异。方法 :分别对表面区、中心区、深层浸润区进行病理分级 (WHO) ,并统计观察其差异性。采用S P免疫组织化学染色方法来检验口腔鳞癌标本的不同区域的p53、p2 1基因蛋白表达差异。结果 :2 0例口腔鳞癌标本中表面区70 %属病理I级 ,无一例Ⅲ级 ,中心区则 65 %属病理Ⅱ级 ,深层浸润区 70 %为病理Ⅲ级 ,无一例I级 ;两两比较 ,差异有显著性 (P <0 .0 1)。p53和p2 1基因蛋白表达亦随病变的区域出现不同阳性表达率。结论 :本研究发现口腔鳞癌在其表面区域、中央区域、深层浸润区域 ,病变的分化程度有明显差异 ,即自表面至中心至浸润区分化程度越来越差。p53和 p2 1基因蛋白的表达亦出现类似趋势 ,即随着病变进展阳性表达率亦随之增高。
OBJECTIVE: To study the pathological grading of different regions and the differences of p53 and p21 gene protein expression by dividing the different surgical specimens of oral squamous cell carcinoma. Methods: The surface area, central area and deep infiltration area were respectively graded by pathology (WHO), and the differences were statistically observed. S P immunohistochemical staining was used to examine the differences in p53 and p21 gene expression in different regions of oral squamous cell carcinoma specimens. Results: Totally 70% of the surface area of 20 oral squamous cell carcinoma specimens were pathological grade I, none of grade III, 65% of pathological grade II in central area, 70% of pathological grade III in deep invaded area, none of grade I; The difference between the two groups was significant (P <0.01). The expression of p53 and p21 gene proteins also showed different positive expression rates along with the lesion area. Conclusion: The study found that oral squamous cell carcinoma in the surface area, central area, deep infiltration area, the degree of differentiation of the lesions were significantly different, that is, from the surface to the center to infiltration zone differentiation worse. The expression of p53 and p21 gene proteins also showed a similar trend, that is, with the progress of the disease the positive expression rate also increased.