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目的观察短链脂肪酸(SCFA)作用下伤寒沙门菌能否诱导巨噬细胞凋亡及其机制。方法将伤寒沙门菌及SCFA作用菌与巨噬细胞共孵育,于2、4、8、12和24h后,用流式细胞术检测细胞凋亡率,并于作用8h后检测NO、TNF-α、caspase-3产生量,及加入TNF-α抗体和caspase-3抑制剂后的细胞凋亡率。结果SCFA作用下的伤寒沙门菌可诱导巨噬细胞凋亡,凋亡率与对照组及伤寒沙门菌原菌组比较差异有统计学意义(P<0.01);作用8h后NO、TNF-α、caspase-3的产生量均高于对照组(P<0.01);加入TNF-α抗体后凋亡率明显降低(P<0.01)。结论SCFA作用下伤寒沙门菌可诱导巨噬细胞凋亡,其凋亡可能由活性氮及细胞因子TNF-α介导和caspase-3参与。
Objective To observe whether Salmonella typhi can induce macrophage apoptosis under the action of short chain fatty acids (SCFA) and its mechanism. METHODS: Salmonella typhimurium and SCFA were incubated with macrophages. After 2, 4, 8, 12 and 24 hours, the apoptotic rates were detected by flow cytometry. After 8 hours, the levels of NO and TNF-α , The production of caspase-3, and the apoptosis rate after adding TNF-α antibody and caspase-3 inhibitor. Results Salmonella typhimurium induced the apoptosis of macrophages under the action of SCFA. The apoptosis rate was significantly different from that of the control group and the Salmonella typhi group (P <0.01), and the levels of NO, TNF-α, The production of caspase-3 was higher than that of the control group (P <0.01). The apoptosis rate was significantly decreased after the addition of TNF-α antibody (P <0.01). Conclusion Salmonella typhimurium induced apoptosis of macrophages under the action of SCFA. The apoptosis may be mediated by active nitrogen and TNF-α and caspase-3.