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目的研究低剂量镉对原代培养的大鼠大脑皮质神经细胞形态及凋亡的影响。方法用新生24 h内SD大鼠,建立了大脑皮质神经细胞原代培养方法,通过不同浓度镉(0、5、10和20μmol/L)染毒12 h后,用倒置相差显微镜观察神经细胞的形态学变化,流式细胞术检测凋亡率和线粒体膜电位。结果与对照组相比,各试验组细胞密度降低,神经网络减少;细胞凋亡率随染毒剂量的增加而升高;线粒体膜电位(ΔΨ)随染毒剂量的增加而降低,当镉浓度为20μmol/L时,差异有统计学意义(P<0.01)。结论在本试验条件下,低剂量镉有抑制神经细胞生长和促进其凋亡的毒性作用。
Objective To study the effect of low-dose cadmium on the morphology and apoptosis of primary cultured rat cerebral cortical neurons. Methods Primary cultures of cerebral cortical neurons were established in neonatal SD rats within 24 h. After exposure to different concentrations of cadmium (0, 5, 10, and 20 μmol/L) for 12 h, the neurons were observed using an inverted phase contrast microscope. Morphological changes, flow cytometry were used to detect apoptosis rate and mitochondrial membrane potential. Results Compared with the control group, the cell density was decreased and the neural network was decreased in each experimental group; the apoptosis rate increased with the increase of the dose; the mitochondrial membrane potential (ΔΨ) decreased with the increase of the dose, when the cadmium concentration At 20 μmol/L, the difference was statistically significant (P<0.01). Conclusion Under the conditions of this experiment, low-dose cadmium has the toxic effect of inhibiting the growth of nerve cells and promoting its apoptosis.